MICROBIOLOGY ATLAS

GUIDE · MICROSCOPES

Buying microscopes for a microbiology teaching lab.

Microscopes are usually the largest single purchase in a teaching lab, and they last for years. This guide helps you settle what you need before you request quotes, so you can compare offers on the things that matter.

This is a planning aid, not a product recommendation. It does not rank brands or models, and nobody pays us for anything on this page. Confirm specifications with your faculty and your institution's procurement and safety staff. How we work.

1. Start with what students need to see

The course decides the optics. Stained bacteria are the deciding case in most microbiology courses: resolving them needs a 100× oil-immersion objective. If any lab exercise involves Gram stains or other stained bacterial smears, every student microscope (or every one used for those exercises) needs one.

If students will…They need
Examine stained bacteria (Gram stain, endospore, acid-fast)100× oil-immersion objective, about 1,000× total magnification, plus a condenser able to match it (see below)
Look at fungi, protozoa or algae in wet mounts40× objective is usually enough; unstained specimens may need phase contrast or careful use of the condenser diaphragm
Observe live, unstained bacteria or motilityPhase contrast or darkfield, which are add-ons with their own cost
Study colonies, plates or larger specimensA stereo (dissecting) microscope, which is a different instrument

Check your lab manual and syllabus against this table before deciding anything else. ASM's curriculum guidelines include microscopy among the laboratory skills students should develop.

2. Work out how many you need

For microscopes, the count is about how many students are at the bench at the same time, not total enrollment.

The item planner does this arithmetic for you: enter the microscopes you need as "units required", the working ones you own as "usable stock", and a pack size of 1.

3. The specification that matters

Most of what separates a frustrating teaching microscope from a good one comes down to a few lines on the quote. Ask every supplier to state each of these.

FeatureWhat to ask forWhy it matters
Objectives4×, 10×, 40× and 100× oil immersion. Ask for the numerical aperture (NA) of each, for example 0.65 for the 40× and about 1.25 for the 100× oil.Resolution depends on NA, not on magnification. A high magnification with a low NA just enlarges blur.
Objective grade"Achromat" is the usual teaching grade. "Plan achromat" gives a field that is sharp to the edges, at higher cost.Plan objectives make it easier for students to see the whole field in focus. Decide whether that is worth the difference.
CondenserAn Abbe condenser with NA 1.25, an iris (aperture) diaphragm and adjustable height.The condenser has to match the 100× objective's NA. A lower-NA condenser limits what that objective can resolve.
Parfocal and parcentered objectivesConfirm both.Students stay in focus and on target when switching objectives, which saves time and slides.
Objective thread standardAsk which standard the objectives use and whether replacements are sold separately.A standard thread means a damaged objective can be replaced on its own instead of the whole microscope.
Eyepieces and head10× wide-field eyepieces; binocular head with interpupillary and diopter adjustment.Binocular heads with adjustment are easier on students who spend a whole lab period at the eyepieces, including those who wear glasses.
IlluminationLED with adjustable intensity.Runs cool and long-lived. Ask about the expected bulb or LED life and replacement cost.
StageMechanical stage with slide holder and coaxial X–Y controls.Lets students scan a smear systematically instead of pushing slides by hand.

Background on numerical aperture and condensers: Evident Scientific, "Numerical Aperture". Oil immersion is needed because air limits a dry objective to an NA below 1.0.

4. Classroom durability

Teaching microscopes are handled by many inexperienced users. These features reduce damage and loss.

5. Service, parts and running costs

6. Optional extras

7. Printable worksheet

Print this page (Ctrl+P or ⌘P) and fill in the table below for each quote you receive. For comparing costs, delivery and terms in more detail, see comparing supplier quotes. Choose "Save as PDF" in the print dialog to keep a digital copy.

RequirementOur needQuote AQuote BQuote C
Supplier and model
Quantity (including spares)
100× oil objective NA≥ 1.25
Condenser NA, iris diaphragm1.25, yes
Objective grade (achromat / plan)
Parfocal and parcenteredYes
Binocular, interpupillary and diopter adjustment
Illumination type and lifeLED
Spring-loaded 40× and 100×Yes
Mechanical stage, coaxial controlsYes
Warranty (years, exclusions)
Repair arrangements
Replacement objective price
Unit price
Freight, tax, other charges
Total quoted cost
Delivery date
Quote valid until

Where to request quotes

Your institution may already have approved suppliers or contract pricing, so check with your purchasing office first. The supplier research section on the planner lists educational suppliers to start with. Those are ordinary, unpaid links.

Last updated 9 October 2026.